Cleaving peptides in flow

Application Note 72

In this application note, we demonstrate how safe, fast, and reliably a peptide is cleaved from a resin in continuous flow. Different cleavage conditions were evaluated using Glucagon-like peptide-1 (GLP-1) as an example peptide. By using the Variable Bed Flow Reactor (VBFR), the packing density of the resin was controlled throughout the reaction, minimising dilution and eliminating the channelling of reagents. Temperature, residence time, and cleavage cocktail were evaluated. The optimised protocol yielded GLP-1 with a crude purity of 82 % in only 30 minutes, compared to a traditional batch reaction taking between 2 to 4 hours.

Application notes
Join the Vapourtec Knowledge Base to view this Application Note