Application Note 72: Cleaving peptides in flow

    VBFR-Mount-50-1482-with-VBFR-Reactor-50-1530

    In this application note, we demonstrate how safe, fast and reliably a peptide is cleaved from a resin in continuous flow. Different cleavage conditions were evaluated using Glucagon-like peptide-1 (GLP-1) as an example peptide. By using the Variable Bed Flow Reactor (VBFR), the packing density of the resin was controlled throughout the reaction, minimising dilution and eliminating channelling of reagents. Temperature, residence time and cleavage cocktail were evaluated. The optimised protocol yielded GLP-1 with a crude purity of 82 % in only 30 minutes, compared to a traditional batch reaction taking between 2 to 4 hours.

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