Peptide Synthesizer overview

The Peptide-Builder is designed for peptide chemists who want to make high quality peptides quickly and easily at laboratory scale. Peptides are synthesized sequentially fully automated either one at a time or queued up to 16, for example overnight.

• Product under development, launch scheduled for Q3 2024
• Synthesis scale 0.05 mmol to 0.20 mmol
• Uses Vapourtec’s patented reactor technology for continuous flow single pass SPPS
• Fmoc solid phase peptide chemistry with DIC and oxyma activation
• Fully automated up to 16 peptides
• Fast cycle times
• Continuous in-line analytics including resin solvation data and UV absorption data
• Automated addition of side chain(s) as required

The Peptide-Explorer is designed for scientists who need to explore and optimise peptide chemistry and ultimately scale-up. The product is a high spec flow chemistry system adapted for continuous flow peptide synthesis and has comprehensive flexibility and analytical capability with proven reliability over 15 years of operation.

• Synthesis scale 0.05 – 1.00 mmol
• Ideal for research
• State of the art peptide synthesis software including sequence generator with automated addition of side chain(s)
• Uses Vapourtec’s patented reactor technology for continuous flow single pass SPPS
• Fmoc solid phase peptide chemistry with DIC and oxyma activation
• Minimises solvent and reagent use compared with competing products
• Continuous in-line analytics including resin solvation data and UV absorption data
• Fast cycle times even at larger scale
• This is a highly flexible product capable of peptide synthesis and continuous flow organic small molecule synthesis including photochemistry and electrochemistry

The Peptide-Scaleup is designed for the peptide chemist aiming to scaleup their peptides where synthesis time is critical. The Peptide-Scaleup is an advanced alternative to traditional room temperature batch scaleup peptide synthesizers. Typically a 30-mer peptide can be synthesized at 4 mmol scale in just 14 hours.

• Synthesis scale 0.5 mmol to 5.0 mmol
• Highest quality crude peptides at large scale, minimises demands on purification
• Heated reactors (VBFR) ensures fast cycle times even at largest scale
• State of the art peptide synthesis software including sequence generator with automated addition of side chain(s)
• Uses Vapourtec’s patented reactor technology (VBFR) for continuous flow single pass SPPS
• Fmoc solid phase peptide chemistry with DIC and oxyma activation
• Minimises solvent and reagent usage compared with competing products
• Continuous in-line analytics including resin solvation data and UV absorption data
• A synthesis optimised on the Peptide-Explorer can be transferred to the Peptide-Pilot without further development

The Peptide-Pilot is a unique peptide synthesizer designed to reduce the drug development cycle time by reducing the time required to produce significant quantities of a target peptide.  Synthesis times are reduced from weeks to less than a day when compared with the normal room temperature batch methods.  Crude purity is excellent even when non-natural amino acids are required.

• Product under development, launch date Q4, 2023
• Synthesis scale 2.0 mmol to 30.0 mmol
• Highest quality crude peptides at large scale
• Heated reactors (VBFR) ensures fast cycle times even at largest scale
• Typical reaction time for a 30-mer peptide at 20 mmol scale is 20 hours
• Uses Vapourtec’s patented reactor technology (VBFR) for continuous flow single pass SPPS
• State of the art peptide synthesis software including sequence generator with automated addition of side chain(s) and real time analysis of synthesis quality
• Fmoc solid phase peptide chemistry with DIC and oxyma activation
• Minimises solvent and reagent usage.  Solvent usage typically less than 180 ml per mmol / cycle even when using PEG resins
• Continuous in-line analytics including resin solvation data and UV absorption data
• A synthesis optimised on the Peptide-Explorer can be transferred to the Peptide-Pilot without further development

Real-time monitoring of solid-phase peptide synthesis using a variable bed flow reactor†

Eric T. Sletten  a, Manuel Nuño  b, Duncan Guthrie b and Peter H. Seeberger  *ac
aDepartment of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany.