Application Note 69 : Automated CF SPPS and evaluation of GLP-1 peptide

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    This application note illustrates the capabilities of a Vapourtec RS-400 system, equipped with a novel flow reactor, the Variable Bed Flow Reactor, for continuous flow Solid-Phase Peptide Synthesis. Glucagon like peptide (GLP-1), a 30-mer peptide used to treat diabetes, was chosen to show the advantages of the Variable Bed Flow Reactor for solid-phase peptide synthesis regardless of the resin core used. By keeping the solid media continuously packed, the contact surface between the resin’s active sites and the reagents pumped through is optimised. The use of the Variable Bed Flow Reactor not only reduces the amount of solvent required, but it also ensures a more efficient synthesis.

    The packing density and overall protocol allows a final crude purity of the 30-mer peptide, GLP-1 up to 82 % in under 5 hours.

    Application Note 69 – Automated CF SPPS and evaluation of GLP-1 peptide

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