Date: 9 September 2024 | Category: News
Vapourtec has developed an online UV detector designed specifically for peptide synthesis and flow chemistry applications.
Rugged, reliable and compact, the Fmoc-UV has no moving parts and features a low-cost flow cell that can be easily replaced in the event of any blockages.
The Fmoc-UV has been designed to quantify the amount of the fluorenylmethoxycarbonyl (Fmoc) protecting group in the outflow during solid-phase peptide synthesis (SPPS) and analyses two wavelengths (365 nm and 460 nm) at an acquisition rate of 4 Hz.
The 365 nm wavelength is aimed at quantifying Fmoc following deprotection of the standard protection group while the 460 nm wavelength is ideal for the 4-methyltrityl (Mtt) group, which is commonly used during the construction of side chains in the synthesis of active pharmaceutical ingredients such as semaglutide and others.
Vapourtec chief scientific officer Manuel Nuño explained: ‘Most UV detectors are designed for the low concentration and relatively non-corrosive solutions used in high-performance liquid chromatography (HPLC) applications.
‘Flow chemistry applications, by contrast, require the use of corrosive reagents, often at high concentrations.
‘Designed for peptide synthesis, the Fmoc-UV is also suitable for other applications in both flow and batch chemistry environments where it can easily perform online sampling of batch reactors,’ Manuel added.
Other key features of the Fmoc-UV include serial communication via RS232 protocols and the ability to interface directly with Vapourtec’s R-Series software.
Learn more about the Fmoc-UV detector
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