Real-Time Monitoring of Solid-Phase Peptide Synthesis Using a Variable Bed Flow Reactor

• Eric T. Sletten^a
• Manuel Nuno^b
• Duncan Guthrie^b
• Peter Seeberger^a,c

• ^aDepartment of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany
• ^bVapourtec Ltd, Park Farm Business Centre, Fornham St Genevieve, Bury St Edmunds, Suffolk IP28 6TS, U.K
• ^cDepartment of Chemistry and Biochemistry, Freie Universität Berlin, Arnimalle 22, 14195, Berlin, Germany

On-resin aggregation and incomplete amide bond formation are major challenges for solid-phase peptide synthesis that are difficult to be monitored in real-time. Incorporation of a pressure-based variable bed flow reactor into an automated solid-phase peptide synthesizer permitted real-time monitoring of resin swelling to determine amino acid coupling efficiency and on-resin aggregation.

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