Mimicking natural metabolisms: cell‐free flow preparation of dopamine

    • Silvia Donzellaa
    • Agostina Colaciccoa
    • Luca Nespolia
    • Dr. Martina L. Contentea
    • aDepartment of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, via Celoria, 2, 20133 Milan, Italy

    The biosynthesis of dopamine (DA) from L-tyrosine as starting material is an excellent yet challenging strategy. Here we developed a versatile, multi-enzymatic platform for the biocatalytic preparation of DA in a continuous mode with excellent conversion (90 %) and reaction time (45 min). The system exploits the immobilization of a decarboxylase from Bacillus pumilis (Fdc) and a tyrosinase from Agaricus bisporus (Tyr), which were combined to mimic the in-vivo synthesis of DA (both primary and secondary metabolisms) giving rise to an efficient strategy with a considerable reduction of process associated costs and environmental impact. To enhance the system automation, an in-line purification via catch-and-release procedure was added.

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