Continuous flow biocatalysis: production and in-line purification of amines by immobilised transaminase from Halomonas elongata

• Matteo Planchestainer^a
• Martina Letizia Contente^ab
• Jennifer Cassidy^a
• Francesco Molinari^b
• Lucia Tamborini^*c
• Francesca Paradisi^*ad

• ^* Corresponding authors
• ^a UCD School of Chemistry, University College Dublin, Belfield, Dublin 4, Ireland
• ^b Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli studi di Milano, Via Mangiagalli 25, Milan, Italy
• ^c Department of Pharmaceutical Sciences (DISFARM), Università degli studi di Milano, Via Mangiagalli 25, Milan, Italy
• ^d School of Chemistry, University of Nottingham, University Park, Nottingham, UK

The continuous flow synthesis of a series of amines was successfully achieved by exploiting the enhanced stability and broad substrate scope of an immobilised transaminase from Halomonas elongata (HEWT). A series of substrates were tested in flow reactors and transformed into the corresponding amines in good to excellent yields. The process was implemented with an integrated in-line purification step for the recovery of the pure amines.

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